Axial resolution of confocal Raman microscopes: Gaussian beam theory and practice

Grauw de, C.J. and Sijtsema, N.M. and Otto, C. and Greve, J. (1997) Axial resolution of confocal Raman microscopes: Gaussian beam theory and practice. Journal of Microscopy, 188 (3). pp. 273-279. ISSN 0022-2720

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Abstract:	A straightforward and transparent model, based on Gaussian beam optics, for the axial r0 resolution of a confocal microscope is presented. A confocal Raman microscope was used to determine the axial confocality in practice. The axial response of a thin planar object was measured for three different objectives, two pinhole sizes and a slit. The results show that, in the case of a confocal configuration, the response calculated with the model provides a good prediction of the axial resolution of the confocal microscope.
Item Type:	Article
Copyright:	© 1997 Wiley InterScience
Faculty:	Science and Technology (TNW)
Research Group:	Medical Cell BioPhysics (MCBP)
Link to this item:	http://purl.utwente.nl/publications/71355
Official URL:	http://dx.doi.org/10.1046/j.1365-2818.1997.2620818.x
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