Investigations on vinylene carbonate. VI. Immobilization of alkaline phosphatase onto poly(vinylene carbonate)-jeffamine® hydrogel beads

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Chen, Guohua and Does van der, Leen and Bantjes, Adriaan (1993) Investigations on vinylene carbonate. VI. Immobilization of alkaline phosphatase onto poly(vinylene carbonate)-jeffamine® hydrogel beads. Journal of Applied Polymer Science, 48 (7). pp. 1189-1198. ISSN 0021-8995

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Abstract:Hydrogel-like polymers with reactive cyclic carbonate (CCA) groups have been obtained by the cross-linking reaction of poly(vinylene carbonate) (PVCA) with Jeffamines®. By this reaction, microspheric hydrogel beads with a high water content and a high concentration of reactive CCA groups were prepared. The beads were used as a matrix for the immobilization of the enzyme alkaline phosphatase (ALP) and showed a considerable capacity to couple ALP and a reasonable retention of activity for the immobilized ALP, depending on the reaction conditions. The immobilized ALP exhibited a better thermal stability than did native ALP and a high residual activity was found after repeated use.
Item Type:Article
Copyright:© 1993 Wiley InterScience
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Link to this item:http://purl.utwente.nl/publications/71043
Official URL:http://dx.doi.org/10.1002/app.1993.070480707
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