Poly(dimethylsiloxane)-poly(ethylene oxide)-heparin block copolymers II: Surface characterization and in vitro assessments

Share/Save/Bookmark

Grainger, D.W. and Knutson, K. and Kim, S.W. and Feijen, J. (1990) Poly(dimethylsiloxane)-poly(ethylene oxide)-heparin block copolymers II: Surface characterization and in vitro assessments. Journal of Biomedical Materials Research, 24 (4). pp. 403-431. ISSN 0021-9304

open access
[img]
Preview
PDF
1MB
Abstract:Amphiphilic block copolymers containing poly(dimethylsiloxane), poly(ethylene oxide), as well as heparin-coated glass beads and tubes were evaluated for the amounts and activities of surface-immobilized heparin. Because the amphiphilic copolymer system is thermodynanmcally predicted to demonstrate low-energy phase enrichment on the surfaces of aircast films, studies were also undertaken to understand the in vitro results. Solvent-cast copolymer films have a heterogeneous microphase-separated structure according to transmission electron micrographs. Wilhelmy plate contact angle analysis indicates significant surface restructuring occurs upon hydration. Attenuated total reflectance infrared spectroscopy studies of the desiccated and hydrated films at two different sampling depths show compositional heterogeneity as a function of depth, as well as near surface restructuring allowing surface enrichment of the high-energy segments following contact with water. Significant concentrations of heparin are detected on the surface of these coatings by toluidine blue assays. In addition, a portion of the surface-bound heparin maintains its original bioactivity as determined by recalification times, thrombin times, and Factor Xa assays. These substrates were also tested for platelet adhesion and activation reactions in vitro using polymer-coated beads in rabbit platelet-rich plasma. Heparinized polymers promoted low levels of platelet adhesion and serotonin release. Surface concentrations of heparin from bioactivity assays were then correlated with platelet adhesion and the extent of platelet release to assess the efficacy of this heparin-immobilized copolymer as a blood-compatible material or coating.
Item Type:Article
Copyright:© 1990 Wiley InterScience
Faculty:
Science and Technology (TNW)
Research Group:
Link to this item:http://purl.utwente.nl/publications/70913
Official URL:http://dx.doi.org/10.1002/jbm.820240402
Export this item as:BibTeX
EndNote
HTML Citation
Reference Manager

 

Repository Staff Only: item control page