Raman microspectroscopy of LiDNA fibres in ethanol and tert-butanol

Mud, J. and Otto, C. and Mul de, F.F.M. and Greve, J. (1985) Raman microspectroscopy of LiDNA fibres in ethanol and tert-butanol. Journal of Raman Spectroscopy, 16 (6). pp. 373-376. ISSN 0377-0486

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Abstract:	The conformation of low-salt LiDNA in fibrous form has been studied by Raman microspectroscopy. The recorded spectrum of a fibre soaked in ethanol is very similar to that of a fibre at low relative humidity. From x-ray experiments it is known that in the latter case the DNA adopts the C conformation. Therefore, it can be concluded that this is also the case in a low-salt LiDNA fibre soaked in ethanol. Further, it has been observed that the DNA conformation did not change when the excess ethanol was allowed to evaporate. The C form is also found when the fibre is in contact with ethanol vapour only. These results are in agreement with x-ray studies. However, our experiments with a LiDNA fibre in tert-butanol are not in agreement with x-ray results. Only when the tert-butanol is absolutely free from water is a state intermediate between the B and C forms found.
Item Type:	Article
Copyright:	© 1985 Wiley InterScience
Faculty:	Science and Technology (TNW)
Research Group:	Medical Cell BioPhysics (MCBP)
Link to this item:	http://purl.utwente.nl/publications/70732
Official URL:	http://dx.doi.org/10.1002/jrs.1250160605
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