Concentration polarization phenomena during dead-end ultrafiltration of protein mixtures. The influence of solute-solute interactions

Berg van den, G.B. and Smolders, C.A. (1989) Concentration polarization phenomena during dead-end ultrafiltration of protein mixtures. The influence of solute-solute interactions. Journal of Membrane Science, 47 (1-2). pp. 1-24. ISSN 0376-7388

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Abstract:	The flux decline behaviour of some charged proteins and of binary mixtures of charged solutes during unstirred dead-end ultrafiltration has been studied. The mixtures consisted of the proteins bovine serum albumin, (BSA), α-lactalbumin and/or lysozyme. Of special interest were α-lactalbumin and lysozyme because these proteins are physico-chemically identical, except for the sign of their charge at the conditions used (pH = 7.4, I=0.125 N and T=20°C). The ultrafiltration properties were studied using the boundary layer resistance model. Ultrafiltration of single protein solutions of α-lactalbumin and of lysozyme showed identical characteristics. The fouling behaviour during ultrafiltration of binary mixtures of the three components appeared to be dependent on both the charge of the solutes and the (unequal) dimensions of the solutes. A mixture of oppositely charged proteins (i.e., BSA/lysozyme or α-lactalbumin/lysozyme) sometimes showed a considerable increase of the resistance of the concentrated layer near the membrane, depending on the mixing ratio of the two proteins. When equally charged (i.e., BSA/α-lactalbumin) proteins are ultrafiltered, a small decrease of the resistance could be observed, again depending on the mixing ratio of the proteins. The charge of the proteins, especially opposite charges, appeared to influence the flux behaviour more than the slightly denser packing of the solutes (as a result of unequal dimensions) would account for.
Item Type:	Article
Copyright:	© 1989 Elsevier
Link to this item:	http://purl.utwente.nl/publications/70474
Official URL:	http://dx.doi.org/10.1016/S0376-7388(00)80857-1
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