Monitoring of DNA molecules in a lab on a chip with femtosecond laser written waveguides


Pollnau, M. and Dongre, C. and Dekker, R. and Hoekstra, H.J.W.M. and Martinez-Vazquez, R. and Osellame, R. and Ramponi, R. and Cerullo, G. and Weeghel, R. van and Besselink, G.A.J. and Vlekkert, H.H. van den (2009) Monitoring of DNA molecules in a lab on a chip with femtosecond laser written waveguides. In: 39th Winter Colloquium on The Physics of Quantum Electronics, January 4-8, 2009, Snowbird, Utah, USA (pp. p. 213).

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Abstract:Using femtosecond laser writing, optical waveguides were monolithically integrated into a commercial microfluidic lab-on-a-chip device, with the waveguides intersecting a microfluidic channel. Continuous-wave laser excitation through these optical waveguides confines the excitation window to a width of 12 um, enabling high-resolution monitoring of the passage of different types of fluorescent analytes, when migrating and being separated in the microfluidic channel by microchip capillary electrophoresis. We demonstrate on-chip-integrated waveguide excitation and detection of a biologically relevant species, fluorescently labeled Deoxyribonucleic acid (DNA) molecules, as well as separation of different dyes, Rhodamine-6G and Rhodamine-B during microchip capillary electrophoresis. Well-controlled plug formation as required for on-chip integrated capillary electrophoresis separation of DNA molecules, and the combination of waveguide excitation and a low detection limit will enable monitoring of extremely small quantities with high spatial resolution.
Item Type:Conference or Workshop Item
Electrical Engineering, Mathematics and Computer Science (EEMCS)
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