Time-dependent changes in ghrelin-immunoreactivity in dissociated neuronal cultures of the newborn rat neocortex

Stoyanova, Irina I. and Wiertz, Remy W.F. and Rutten, Wim L.C. (2009) Time-dependent changes in ghrelin-immunoreactivity in dissociated neuronal cultures of the newborn rat neocortex. Regulatory peptides, 158 (1-3). pp. 86-90. ISSN 0167-0115

PDF
Restricted to UT campus only: Request a copy
1138Kb

Abstract:	Ghrelin is a hormone, initially described as a gastric peptide stimulating appetite and growth hormone secretion, which also has an important role in the regulation of many other processes, including higher brain functions. Ghrelin has been described in situ in different parts of the brain, but so far there has been no data about its expression in cell cultures. Therefore, we aimed in this study to investigate the levels of ghrelin in dissociated cortical neurons at various times in culture. We applied the ABC immunocytochemical method for the detection of ghrelin in one-day-, one-week-, and two-week-old cultures. Our results clearly show that at the early stages after plating the cultures 86.2% (± 8.93) of the neurons are ghrelin-positive and their number decreases during the culturing period. As ghrelin is present in the majority of cultured newborn neurons, when the neuronal differentiation and network formation take place, it may also influence the early synaptic formation and cell-to-cell interactions, which are both very important for network functions like learning and memory.
Item Type:	Article
Copyright:	© 2009 Elsevier
Faculty:	Electrical Engineering, Mathematics and Computer Science (EEMCS)
Research Group:	Biomedical Signals and Systems (BSS)
Link to this item:	http://purl.utwente.nl/publications/70130
Official URL:	http://dx.doi.org/10.1016/j.regpep.2009.06.008
Export this item as:	BibTeX EndNote HTML Citation Reference Manager

Show download statistics for this publication

Repository Staff Only: item control page

Metis ID: 266485