DNA Separation and Fluorescence Monitoring by Integrated Waveguides in an Optofluidic Chip

Dongre, C. and Weerd van, J. and Besselink, G.A.J. and Martinez Vazquez, R. and Osellame, R. and Ramponi, R. and Cerullo, G. and Weeghel van, R. and Vlekkert van den, H.H. and Hoekstra, H.J.W.M. and Pollnau, M. (2009) DNA Separation and Fluorescence Monitoring by Integrated Waveguides in an Optofluidic Chip. In: 2009 Annual Symposium of the IEEE Photonics Benelux Chapter, 5-6 November 2009, Brussels, Belgium.

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Abstract:	We report on the monolithic integration of optical waveguides and microfluidics in a fused-silica lab-on-a-chip. Labeled biomolecules such as double-stranded DNA are flown and separated in the microfluidic channel by capillary electrophoresis and their fluorescent labels are excited by a continuous-wave laser beam through femtosecondlaser-written integrated waveguides. In this context, desirable features such as high spatial resolution (~12 μm), and a low limit of detection (~ 6 nano-molar) have been experimentally demonstrated. The proof of concept is being extended to real-world diagnostic samples for on-chip diagnosis of genetic diseases, e.g. breast cancer.
Item Type:	Conference or Workshop Item
Faculty:	Electrical Engineering, Mathematics and Computer Science (EEMCS)
Research Group:	Integrated Optical MicroSystems (IOMS)
Link to this item:	http://purl.utwente.nl/publications/69941
Organisation URL:	http://www.photonics-benelux.org
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