Parallel single cell analysis on an integrated microfluidic platform for cell trapping, lysis and analysis


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Le Gac, S. and Boer, H. de and Wijnperle, D. and Meuleman, W. and Carlen, E.T. and Berg, A. van den (2009) Parallel single cell analysis on an integrated microfluidic platform for cell trapping, lysis and analysis. In: MicroTAS 2009, Thirteenth International Conference on Miniaturized Systems for Chemistry and Life Sciences, 1-5 Nov 2009, Jeju, Korea (pp. pp. 1808-1810).

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Abstract:We report here a novel and easily scalable microfluidic platform for the parallel analysis of hundreds of individual cells, with controlled single cell trapping, followed by their lysis and subsequent retrieval of the cellular content for on-chip analysis. The device consists of a main channel and an array of shallow side channels connected to the main channel via trapping structures. Cells are individually captured in dam structures by application of a negative pressure from an outlet reservoir, lyzed on site and the cellular content controllably extracted and transported in the individual side channels for on-chip analysis.
Item Type:Conference or Workshop Item
Copyright:© 2009 CBMS
Faculty:
Electrical Engineering, Mathematics and Computer Science (EEMCS)
Research Group:
Link to this item:http://purl.utwente.nl/publications/69835
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