Competitive adsorption of plasma proteins at solid—liquid interfaces


Lensen, H.G.W. and Breemhaar, W. and Smolders, C.A. and Feijen, J. (1986) Competitive adsorption of plasma proteins at solid—liquid interfaces. Journal of Chromatography B: Biomedical Sciences and Applications, 376 . pp. 191-198. ISSN 1570-0232

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Abstract:The competitive adsorption of human serum albumin (HSA), human immuno-γ-globulin (HIgG) and human fibrinogen (HFb) onto polystyrene (PS) at 20° C and a pH of 7.35 (phosphate-buffered saline) was studied. Protein adsorption was studied using enzyme immunoassay. The results obtained with the immunoassay were compared with those obtained using radiolabelled proteins. Recent studies revealed that the adsorption behaviour of radiolabelled proteins onto surfaces differs from that of the non-labelled proteins, which may lead to misinterpretation of adsorption data. Differences in the adsorption behaviour of the labelled proteins as compared to non-labelled proteins can possibly be explained by the formation of modified proteins during the labelling procedure as shown by ion-exchange high-performance liquid chromatography (HPLC). The competitive adsorption of HSA, HIgG and HFb onto a PS latex was studied by measuring the depletion of proteins in solution. The decrease in protein concentration in solution was determined by HPLC techniques. A strong preferential adsorption of HFb was observed with maximum adsorption values of 0.6 μg/cm2.
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Copyright:© 1986 Elsevier Science
Science and Technology (TNW)
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