The adsorption behaviour of two commercial IgG-preparations onto a polystyrene latex surface

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Ronner, J.A. and Lensen, H.G.W. and Olthuis, P.M.F.G. and Smolders, C.A. and Feijen, J. (1984) The adsorption behaviour of two commercial IgG-preparations onto a polystyrene latex surface. Biomaterials, 5 (4). p. 241. ISSN 0142-9612

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Abstract:The adsorption behaviour of two commercial preparations of human IgG onto a polystyrene latex surface was studied. The adsorption isotherms obtained differed markedly, one preparation showed a plateau value of 0.4 μg cm−2 which was reached at 0.1g I−1, whereas the other preparation showed no plateau value within the concentration range studied (0.1–7.0 g I−1). Characterization by means of iso-electric focusing and HPLC also showed differences between the two preparations. No differences were observed when immuno-electrophoresis was carried out These results stress the necessity for proper characterization of proteins used in adsorption studies.
Item Type:Article
Copyright:© 1984 Elsevier Science
Faculty:
Science and Technology (TNW)
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Link to this item:http://purl.utwente.nl/publications/69354
Official URL:http://dx.doi.org/10.1016/0142-9612(84)90023-1
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