Novel poly(amido amine)s with bioreducible disulfide linkages in their diamino-units: Structure effects and in vitro gene transfer properties

Piest, M. and Lin, Chao and Mateos Timoneda, M.A. and Lok, M.C. and Hennink, W.E. and Feijen, J. and Engbersen, J.F.J. (2008) Novel poly(amido amine)s with bioreducible disulfide linkages in their diamino-units: Structure effects and in vitro gene transfer properties. Journal of Controlled Release, 132 (3). e12-e13. ISSN 0168-3659

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Abstract:	A series of novel water-soluble, bioreducible poly(amido amine)s (SS-PAAs) was synthesized by Michael addition polymerization of N,N′-dimethylcystamine (DMC) with various bisacrylamides. DMC-based SS-PAAs show high buffer capacities in the pH range 5.1–7.4, facilitating endosomal escape. The polymers form nanoscaled (< 250 nm) and positively charged (> + 20 mV) polyplexes which are relatively stable, but rapidly disintegrate in the presence of 2.5 mM DTT. These polyplexes have transfection efficiencies up to 4 times higher than bPEI in COS-7 cells, with low cytotoxicity.
Item Type:	Article
Copyright:	© 2008 Elsevier
Faculty:	Science and Technology (TNW)
Research Group:	Polymer Chemistry and BioMaterials (PBM) Biomedical Chemistry (BMC)
Link to this item:	http://purl.utwente.nl/publications/69320
Official URL:	http://dx.doi.org/10.1016/j.jconrel.2008.09.047
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