Novel poly(amido amine)s with bioreducible disulfide linkages in their diamino-units: Structure effects and in vitro gene transfer properties

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Piest, M. and Lin, Chao and Mateos Timoneda, M.A. and Lok, M.C. and Hennink, W.E. and Feijen, J. and Engbersen, J.F.J. (2008) Novel poly(amido amine)s with bioreducible disulfide linkages in their diamino-units: Structure effects and in vitro gene transfer properties. Journal of Controlled Release, 132 (3). e12-e13. ISSN 0168-3659

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Abstract:A series of novel water-soluble, bioreducible poly(amido amine)s (SS-PAAs) was synthesized by Michael addition polymerization of N,N′-dimethylcystamine (DMC) with various bisacrylamides. DMC-based SS-PAAs show high buffer capacities in the pH range 5.1–7.4, facilitating endosomal escape. The polymers form nanoscaled (< 250 nm) and positively charged (> + 20 mV) polyplexes which are relatively stable, but rapidly disintegrate in the presence of 2.5 mM DTT. These polyplexes have transfection efficiencies up to 4 times higher than bPEI in COS-7 cells, with low cytotoxicity.
Item Type:Article
Copyright:© 2008 Elsevier
Faculty:
Science and Technology (TNW)
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Link to this item:http://purl.utwente.nl/publications/69320
Official URL:http://dx.doi.org/10.1016/j.jconrel.2008.09.047
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