High-performance liquid chromatography as a technique to measure the competitive adsorption of plasma proteins onto latices

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Lensen, Henny G.W. and Bargeman, Dick and Bergveld, Piet and Smolders, Cees A. and Feijen, Jan (1984) High-performance liquid chromatography as a technique to measure the competitive adsorption of plasma proteins onto latices. Journal of Colloid and Interface Science, 99 (1). pp. 1-8. ISSN 0021-9797

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Abstract:Isotherms of human serum albumin (HSA), human immunoglobulin G (HIgG), and human fibrinogen (HFb) onto a polystyrene (PS)-latex were determined by depletion of protein in the solution, which was either followed by radioactivity measurements or by UV spectroscopy. Different adsorption isotherms for the same protein were obtained when either radioactivity measurements or UV spectroscopy was used as a detection technique. In order to obtain reliable results from competitive protein adsorption experiments, a method based on the use of high-performance liquid chromatography was developed. A strong preferential adsorption of HFb was observed when adsorption studies were carried out with mixtures of HSA, HFb, and HIgG. When adsorption studies were carried out with solutions containing HSA monomer and dimer, a strong preferential adsorption of HSA dimer was also observed.
Item Type:Article
Copyright:© 1984 Elsevier Science
Faculty:
Science and Technology (TNW)
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Link to this item:http://purl.utwente.nl/publications/69270
Official URL:http://dx.doi.org/10.1016/0021-9797(84)90079-1
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