On-chip electrokinetic sample focusing for microarray-based biomolecular interaction assays


Krishnamoorthy, Ganeshram and Carlen, Edwin T. and Kohlheyer, Dietrich and Schasfoort, Richard and Berg, Albert van den (2008) On-chip electrokinetic sample focusing for microarray-based biomolecular interaction assays. In: 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTas 2008, 12-16 October 2008, San Diego, CA, USA (pp. pp. 311-313).

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Abstract:Label-free measurement techniques, such as surface plasmon resonance (SPR) and surface plasmon resonance imaging (iSPR), are emerging as important complementary methods to conventional bioassays because real-time binding kinetics information can be determined[1]. Recent advances in iSPR are becoming increasingly important to label-free microarray-based assay applications, where multiple biomolecular interactions can be measured simultaneously. However, conventional iSPR microarray assay approaches rely on protein printing techniques for immobilization of the ligand to the gold imaging surface[2]. The combination of microfluidics with iSPR allows the in-situ immobilization of ligands and subsequent analyte transport for the biomolecular interaction assay 3]. In this work, we present an integrated microfluidics and iSPR platform, where ligand immobilization and subsequent sample solutions are precisely positioned with an electroosmotically driven address-flow guiding technique.
Item Type:Conference or Workshop Item
Electrical Engineering, Mathematics and Computer Science (EEMCS)
Science and Technology (TNW)
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