A microfluidic chip for electrochemical conversions in drug metabolism studies


Odijk, M. and Baumann, A. and Lohmann, W. and Brink, F.T.G. van den and Olthuis, W. and Karst, U. and Berg, A. van den (2009) A microfluidic chip for electrochemical conversions in drug metabolism studies. Lab on a chip, 9 (12). pp. 1687-1693. ISSN 1473-0197

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Abstract:We have designed a microfluidic microreactor chip for electrochemical conversion of analytes, containing a palladium reference electrode and platinum working and counter electrodes. The counter electrode is placed in a separate side-channel on chip to prevent unwanted side-products appearing in the measured spectrograms. Using this chip, cyclic voltammograms are measured in volumes of 9.6 nL. Furthermore the conversion efficiency of ferricyanide is characterized using UV/vis-spectroscopy. We have obtained an on-line conversion of 97% using a flow rate of 1 mu L/min. We have used the microreactor chip to study the electrochemical metabolism pathway of amodiaquine using electrochemistry (EC)-liquid chromatography (LC)-mass spectrometry (MS). We have compared our results with measurements obtained with commercially available electrochemical flow-through cells. Using our chip it was possible to obtain similar results. Therefore, we have fabricated an electrochemical cell on-chip which is used successfully in EC-UV/vis and EC-LC-MS experiments.
Item Type:Article
Copyright:© 2009 Royal Society of Chemistry
Electrical Engineering, Mathematics and Computer Science (EEMCS)
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Link to this item:http://purl.utwente.nl/publications/68301
Official URL:http://dx.doi.org/10.1039/b822962g
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