A microfluidic chip for electrochemical conversions in drug metabolism studies

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Odijk, M. and Baumann, A. and Lohmann, W. and Brink van den, F.T.G. and Olthuis, W. and Karst, U. and Berg van den, A. (2009) A microfluidic chip for electrochemical conversions in drug metabolism studies. Lab on a Chip, 9 (12). pp. 1687-1693. ISSN 1473-0197

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Abstract:We have designed a microfluidic microreactor chip for electrochemical conversion of analytes, containing a palladium reference electrode and platinum working and counter electrodes. The counter electrode is placed in a separate side-channel on chip to prevent unwanted side-products appearing in the measured spectrograms. Using this chip, cyclic voltammograms are measured in volumes of 9.6 nL. Furthermore the conversion efficiency of ferricyanide is characterized using UV/vis-spectroscopy. We have obtained an on-line conversion of 97% using a flow rate of 1 mu L/min. We have used the microreactor chip to study the electrochemical metabolism pathway of amodiaquine using electrochemistry (EC)-liquid chromatography (LC)-mass spectrometry (MS). We have compared our results with measurements obtained with commercially available electrochemical flow-through cells. Using our chip it was possible to obtain similar results. Therefore, we have fabricated an electrochemical cell on-chip which is used successfully in EC-UV/vis and EC-LC-MS experiments.
Item Type:Article
Copyright:© 2009 Royal Society of Chemistry
Faculty:
Electrical Engineering, Mathematics and Computer Science (EEMCS)
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Link to this item:http://purl.utwente.nl/publications/68301
Official URL:http://dx.doi.org/10.1039/b822962g
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