Experimental Investigation of the Band Broadening Arising from Short-Range Interchannel Heterogeneities in Chromatographic Beds under the Condition of Identical External Porosity

Share/Save/Bookmark

Eghbali, Hamed and Verdoold, Vincent and Vankeerberghen, Lieselot and Gardeniers, Han and Desmet, Gert (2009) Experimental Investigation of the Band Broadening Arising from Short-Range Interchannel Heterogeneities in Chromatographic Beds under the Condition of Identical External Porosity. Analytical Chemistry, 81 (2). pp. 705-715. ISSN 0003-2700

[img]PDF
Restricted to UT campus only
: Request a copy
1030Kb
Abstract:The interesting possibilities offered by micropillar array columns to investigate the relation between bed heterogeneity and band broadening in chromatographic columns are illustrated and investigated. The perfect control over the microscopic bed structure offered by the photolithographic fabrication technique could be used to produce a heterogeneous bed displaying a short-range interchannel heterogeneity and having exactly the same particle size and external porosity as the perfectly ordered bed that was used as the reference system. According to this approach, any observed difference in band broadening could be directly owed to the effect of the bed heterogeneity. Knowing that the only difference between the investigated beds was their degree of heterogeneity, the obtained plate height measurements provided a unique test to distinguish among the abilities to recognize short-range interchannel heterogeneity of the different existing plate height models. Interpreting the data with the classical van Deemter and Knox models, it was found that the increased bed heterogeneity leads not only to an increase in the A-term constant but also to a clear increase in the C-term constant.
Item Type:Article
Copyright:© 2009 American Chemical Society
Faculty:
Science and Technology (TNW)
Research Group:
Link to this item:http://purl.utwente.nl/publications/67275
Official URL:http://dx.doi.org/10.1021/ac802124p
Export this item as:BibTeX
EndNote
HTML Citation
Reference Manager

 

Repository Staff Only: item control page

Metis ID: 253782