The effect of PEGT/PBT scaffold architecture on the composition of tissue engineered cartilage

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Malda, J. and Woodfield, T.B.F. and Vloodt, F. van der and Wilson, C. and Martens, D.E. and Tramper, J. and Blitterswijk, C.A. van and Riesle, J. (2005) The effect of PEGT/PBT scaffold architecture on the composition of tissue engineered cartilage. Biomaterials, 26 (1). pp. 63-72. ISSN 0142-9612

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Abstract:A highly interconnecting and accessible pore network has been suggested as one of a number of prerequisites in the design of scaffolds for tissue engineering. In the present study, two processing techniques, compression-molding/particulate-leaching (CM), and 3D fiber deposition (3DF), were used to develop porous scaffolds from biodegradable poly(ethylene glycol)-terephthalate/poly(butylene terephthalate) (PEGT/PBT) co-polymers with varying pore architectures. Three-dimensional micro-computed tomography (μCT) was used to characterize scaffold architectures and scaffolds were seeded with articular chondrocytes to evaluate tissue formation. Scaffold porosity ranged between 75% and 80%. Average pore size of tortuous CM scaffolds (182 μm) was lower than those of organized 3DF scaffolds (525 μm). The weight ratio of glycosaminoglycans (GAG)/DNA, as a measure of cartilage-like tissue formation, did not change after 14 days of culture whereas, following subcutaneous implantation, GAG/DNA increased significantly and was significantly higher in 3DF constructs than in CM constructs, whilst collagen type II was present within both constructs. In conclusion, 3DF PEGT/PBT scaffolds create an environment in vivo that enhances cartilaginous matrix deposition and hold particular promise for treatment of articular cartilage defects.
Item Type:Article
Copyright:© 2005 Elsevier Science
Faculty:
Science and Technology (TNW)
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Link to this item:http://purl.utwente.nl/publications/67209
Official URL:http://dx.doi.org/10.1016/j.biomaterials.2004.02.046
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