Porous hybrid structures based on P(DLLA-co-TMC) and collagen for tissue engineering of small-diameter blood vessels

Buttafoco, Laura and Boks, Niels P. and Engbers-Buijtenhuijs, P. and Grijpma, Dirk W. and Poot, André A. and Dijkstra, Piet J. and Vermes, István and Feijen, Jan (2006) Porous hybrid structures based on P(DLLA-co-TMC) and collagen for tissue engineering of small-diameter blood vessels. Journal of Biomedical Materials Research, 79B (2). pp. 425-434. ISSN 0021-9304

PDF
Restricted to UT campus only: Request a copy
341Kb

Abstract:	Poly (D,L-lactide)-7co-(1,3-trimethylene carbonate) [P(DLLA-co-TMC)] (83 mol % DLLA) was used to produce matrices suitable for tissue engineering of small-diameter blood vessels. The copolymer was processed into tubular structures with a porosity of 98% by melt spinning and fiber winding, thus obviating the need of organic solvents that may compromise subsequent cell culture. Unexpectedly, incubation in culture medium at 37°C resulted in disconnection of the contact points between the polymer fibers. To improve the structural stability of these P(DLLA-co-TMC) scaffolds, a collagen microsponge was formed inside the pores of the synthetic matrix by dip coating and freeze drying. Hybrid structures with a porosity of 97% and an average pore size of 102 m were obtained. Structural stability was preserved during incubation in culture medium at 37°C. Smooth-muscle cells (SMCs) were seeded in these hybrid scaffolds and cultured under pulsatile flow conditions in a bioreactor (120 beats/min, 80-120 mmHg). After 7 days of culture in a dynamic environment viable SMCs were homogeneously distributed throughout the constructs, which were five times stronger and stiffer than noncultured scaffolds. Values for yield stress (2.8 ± 0.6 MPa), stiffness (1.6 ± 0.4 MPa), and yield strain (120% ± 20%) were comparable to those of the human artery mesenterica.
Item Type:	Article
Copyright:	© 2006 Wiley Periodicals
Faculty:	Science and Technology (TNW) Electrical Engineering, Mathematics and Computer Science (EEMCS)
Research Group:	Biomaterials Science and Technology (BST) Polymer Chemistry and BioMaterials (PBM) Biomedical and Environmental Sensor systems (BIOS)
Link to this item:	http://purl.utwente.nl/publications/67196
Official URL:	http://dx.doi.org/10.1002/jbm.b.30557
Export this item as:	BibTeX EndNote HTML Citation Reference Manager

Show download statistics for this publication

Repository Staff Only: item control page

Metis ID: 236835