Microchip capillary electrophoresis for point-of-care analysis of lithium

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Vrouwe, Elwin X. and Luttge, Regina and Vermes, Istvan and Berg van den, Albert (2007) Microchip capillary electrophoresis for point-of-care analysis of lithium. Clinical Chemistry, 53 (1). pp. 117-123. ISSN 0009-9147

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Abstract:Background: Microchip capillary electrophoresis (CE) is a promising method for chemical analysis of complex samples such as whole blood. We evaluated the method for point-of-care testing of lithium.
Methods: Chemical separation was performed on standard glass microchip CE devices with a conductivity detector as described in previous work. Here we demonstrate a new sample-to-chip interface. Initially, we took a glass capillary as a sample collector for whole blood from a finger stick. In addition, we designed a novel disposable sample collector and tested it against the clinical standard at the hospital (Medisch Spectrum Twente). Both types of collectors require < 10 mu L of test fluid. The collectors contain an integrated filter membrane, which prevents the transfer of blood cells into the microchip. The combination of such a sample collector with microchip CE allows point-of-care measurements without the need for off-chip sample treatment. This new on-chip protocol was verified against routine lithium testing of 5 patients in the hospital.

Results: Sodium, lithium, magnesium, and calcium were separated in < 20 s. The detection limit for lithium was 0.15 mmol/L.

Conclusions: The new microchip CE system provides a convenient and rapid method for point-of-care testing of electrolytes in serum and whole blood. (c) 2007 American Association for Clinical Chemistry

Item Type:Article
Copyright:© 2007 American Association for Clinical Chemistry
Faculty:
Science and Technology (TNW)
Electrical Engineering, Mathematics and Computer Science (EEMCS)
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Link to this item:http://purl.utwente.nl/publications/64004
Official URL:http://dx.doi.org/10.1373/clinchem.2007.073726
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