Dual role of calbindin-D28K in vesicular catecholamine release from mouse chromaffin cells

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Westerink, R.H.S. and Rook, M.B. and Beekwilder, J.P. and Wadman, W.J. (2006) Dual role of calbindin-D28K in vesicular catecholamine release from mouse chromaffin cells. Journal of Neurochemistry, 99 (2). pp. 628-640. ISSN 0022-3042

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Abstract:Calbindin-D28K is suggested to play a postsynaptic role in
neurotransmission and in the regulation of the intracellular
Ca2+ concentration. However, it is still unclear whether calbindin-
D28K has a role in the regulation of exocytosis, either as
Ca2+ buffer or as Ca2+ sensor. Amperometric recordings of
catecholamine exocytosis from wild-type and calbindin-D28K
knockout mouse chromaffin cells reveal a strong reduction in
the number of released vesicles, as well as in the amount of
neurotransmitter released per fusion event in knockout cells.
However, Ca2+ current recordings and Ca2+ imaging experiments,
including video-rate confocal laser scanning microscopy,
revealed that the intracellular Ca2+ dynamics are
remarkably similar in wild-type and knockout cells. The combined
results demonstrate that calbindin-D28K plays an
important and dual role in exocytosis, affecting both release
frequency and quantal size, apparently without strong effects
on intracellular Ca2+ dynamics. Consequently, the possibility
that calbindin-D28K functions not only as a Ca2+ buffer but
also as a modulator of vesicular catecholamine release is
discussed.
Keywords: amperometry, Ca2+ dynamics, Ca2+ sensor, calcium
binding proteins, exocytosis, video-rate confocal laser
scanning microscopy.
Item Type:Article
Copyright:© 2006 Wiley InterScience
Faculty:
Electrical Engineering, Mathematics and Computer Science (EEMCS)
Research Group:
Link to this item:http://purl.utwente.nl/publications/63766
Official URL:http://dx.doi.org/10.1111/j.1471-4159.2006.04099.x
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