Fluorescence monitoring of microchip capillary electrophoresis separation with monolithically integrated waveguides

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Dongre, C. and Dekker, R. and Hoekstra, H.J.W.M. and Pollnau, M. and Martinez-Vazquez, R. and Osellame, R. and Cerullo, G. and Ramponi, R. and Weeghel van, R. and Besselink, G.A.J. and Vlekkert van den, H. (2008) Fluorescence monitoring of microchip capillary electrophoresis separation with monolithically integrated waveguides. Optics Letters, 33 (21). pp. 2503-2505. ISSN 0146-9592

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Abstract:Using femtosecond laser writing, optical waveguides were monolithically integrated into a commercial microfluidic lab-on-a-chip device, with the waveguides intersecting a microfluidic channel. Continuous-wave laser excitation through these optical waveguides confines the excitation window to a width of 12 μm, enabling high-resolution monitoring of the passage of different types of fluorescent analytes when migrating and being separated in the microfluidic channel by microchip capillary electrophoresis. Furthermore, we demonstrate on-chip-integrated waveguide excitation and detection of a biologically relevant species, fluorescently labeled DNA molecules, during microchip capillary electrophoresis. Well-controlled plug formation as required for on-chip integrated capillary electrophoresis separation of DNA molecules, and the combination of waveguide excitation and a low limit of detection, will enable monitoring of extremely small quantities with high spatial resolution.
Item Type:Article
Copyright:© 2008 Optical Society of America
Faculty:
Electrical Engineering, Mathematics and Computer Science (EEMCS)
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Link to this item:http://purl.utwente.nl/publications/62583
Official URL:http://dx.doi.org/10.1364/OL.33.002503
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Metis ID: 254953