Integrated microfluidic system enabling (bio)chemical reactions with on-line MALDI-TOF mass spectrometry


Brivio, Monica and Fokkens, Roel H. and Verboom, Willem and Reinhoudt, David N. and Tas, Niels R. and Goedbloed, Martijn and Berg, Albert van den (2002) Integrated microfluidic system enabling (bio)chemical reactions with on-line MALDI-TOF mass spectrometry. Analytical Chemistry, 74 (16). pp. 3972-3976. ISSN 0003-2700

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Abstract:A continuous flow micro total analysis system (μ-TAS) consisting of an on-chip microfluidic device connected to a matrix assisted laser desorption ionization [MALDI] time-of-flight [TOF] mass spectrometer (MS) as an analytical screening system is presented. Reaction microchannels and inlet/outlet reservoirs were fabricated by powderblasting on glass wafers that were then bonded to silicon substrates. The novel lab-on-a-chip was realized by integrating the microdevice with a MALDI-TOFMS standard sample plate used as carrier to get the microfluidic device in the MALDI instrument. A novel pressure-driven pumping mechanism using the vacuum of the instrument as a driving force induces flow in the reaction microchannel in a self-activating way. Organic syntheses as well as biochemical reactions are carried out entirely inside the MALDI-MS ionization vacuum chamber and analyzed on-line by MALDI-TOFMS in real time. The effectiveness of the μ-TAS system has been successfully demonstrated with several examples of (bio)chemical reactions.
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Copyright:© 2002 American Chemical Society
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Electrical Engineering, Mathematics and Computer Science (EEMCS)
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