Two-photon fluorescence excitation using an integrated optical microcavity: a promising tool for biosensing of natural chromophores

Krioukov, Evgueni and Klunder, Dion and Driessen, Alfred and Greve, Jan and Otto, Cees (2005) Two-photon fluorescence excitation using an integrated optical microcavity: a promising tool for biosensing of natural chromophores. Talanta, 65 (5). pp. 1086-1090. ISSN 0039-9140

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Abstract:	Application of an integrated optics (IO) microcavity (MC) for evanescent excitation of two-photon excited fluorescence (TPF) is demonstrated. The MC provides a high local intensity, which is required for the TPF, because of resonant enhancement of the intracavity power and a strong two-dimensional confinement of the guided mode. Numerical estimations show a large increase, by more than a factor of 104 of the TPF intensity at the MC compared to a conventional straight waveguide. This will lead to a significant improvement of the detection limits of UV-absorbing chromophores (down to 10−8 M) when using the MC as a biosensor. Feasibility of TPF excitation using an IO MC is confirmed experimentally for the first time.
Item Type:	Article
Copyright:	© 2005 Elsevier Science
Faculty:	Science and Technology (TNW)
Research Group:	Medical Cell BioPhysics (MCBP)
Link to this item:	http://purl.utwente.nl/publications/62263
Official URL:	http://dx.doi.org/10.1016/j.talanta.2004.02.048
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