Biomolecular Interaction Monitoring of Autoantibodies by Scanning Surface Plasmon Resonance Microarray Imaging

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Lokate, Angelique M.C. and Beusink, J. Bianca and Besselink, Geert A.J. and Pruijn, Ger J.M. and Schasfoort, Richard B.M. (2007) Biomolecular Interaction Monitoring of Autoantibodies by Scanning Surface Plasmon Resonance Microarray Imaging. Journal of the American Chemical Society, 129 (45). pp. 14013-14018. ISSN 0002-7863

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Abstract:A new commercial surface plasmon resonance (SPR) imaging analysis system with a novel SPR dip angle scanning principle allows the measurement, without the need for labeling, of the exact SPR dip angle. With this system hundreds of biomolecular interactions can be monitored on microarrays simultaneously and with great precision. The potency of this system is demonstrated by automatically monitoring the interactions between citrullinated peptides and serum autoantibodies of 50 rheumatoid arthritis (RA) patients and 29 controls in a single step. The smallest antibody concentration that could be measured in this experimental setup was 0.5 pM.
Item Type:Article
Copyright:© 2007 American Chemical Society
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Science and Technology (TNW)
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Link to this item:http://purl.utwente.nl/publications/62134
Official URL:http://dx.doi.org/10.1021/ja075103x
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Metis ID: 245967