DNA movement in sub-20 nm nanoslits


Share/Save/Bookmark

Salieb-Beugelaar, Georgette and Teapal, Juliane and Nieuwkasteele van, Jan and Wijnperle, Daniël and Tegenfeldt, Jonas O. and Eijkel, Jan C.T. and Berg van den, Albert (2007) DNA movement in sub-20 nm nanoslits. In: Eleventh International Conference on Miniaturized Systems for Chemistry and Life Sciences - The proceedings of microTAS 2007 Conference, 7-11 october 2007, Paris, France.

[img]PDF
Restricted to UT campus only
: Request a copy
104Kb
Abstract:The movement of XbaI digested -DNA in 20 nanomometer and .. -DNA in 12 nanometer high slits was investigated. We found that DNA moved intermittently and following preferential pathways, indicating an important influence of surface roughness. From these mobilities and averaged mobilities between the intermittent sticking events. The friction coefficient per unit length was calculated from the latter mobilities. A three order of magnitude increase was found for the 12 nm slits compared to the theoretical value. The mobility furthermore differs less than one order of magnitude between 20 nm and 12 nm slits, and the influence of varying the ionic strength of the buffer was not significant. This work is the first time DNA movement in such shallow constrictions is investigated.
Item Type:Conference or Workshop Item
Faculty:
Science and Technology (TNW)
Electrical Engineering, Mathematics and Computer Science (EEMCS)
Research Group:
Link to this item:http://purl.utwente.nl/publications/62101
Export this item as:BibTeX
EndNote
HTML Citation
Reference Manager

 

Repository Staff Only: item control page

Metis ID: 245922