Physical discrimination between human T-lymphocyte subpopulations by means of light scattering, revealing two populations of T8-positive cells

Terstappen, L.W.M.M. and Grooth de, B.G. and Nolten, G.M.J. and Napel ten, C.H.H. and Berkel van, W. and Greve, J. (1986) Physical discrimination between human T-lymphocyte subpopulations by means of light scattering, revealing two populations of T8-positive cells. Cytometry Part A, 7 (2). pp. 178-183. ISSN 0196-4763

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Abstract:	Light-scattering properties of human T-lymphocyte subpopulations selected by immunofluorescence were studied. Based on differences in orthogonal light scattering, two subpopulations of T8-positive cells can be distinguished. The first population (T8a) has the same orthogonal light-scattering properties as T4-positive cells, whereas the orthogonal light scattering of the second population (T8b) was about 70% larger. Orthogonal light scattering of Leu7-positive lymphocytes resembles that of the T8b population. We have studied the occurrence of the subpopulation in healthy individuals and we discuss their possible functional identification. Light-scattering properties of lymphocyte subpopulations in two patients with B-cell chronic lymphatic leukemia suggest that this observation is of clinical interest.
Item Type:	Article
Copyright:	© 1986 Wiley
Faculty:	Science and Technology (TNW)
Research Group:	Medical Cell BioPhysics (MCBP)
Link to this item:	http://purl.utwente.nl/publications/60748
Official URL:	http://dx.doi.org/10.1002/cyto.990070209
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