Flow cytometric determination of circulating immune complexes with the indirect granulocyte phagocytosis test

Terstappen, L.W.M.M. and Grooth de, B.G. and Nolten, G.M.J. and Napel ten, C.H.H. and Berkel van, W. and Greve, J. (1985) Flow cytometric determination of circulating immune complexes with the indirect granulocyte phagocytosis test. Cytometry Part A, 6 (4). pp. 316-320. ISSN 0196-4763

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Abstract:	A method for the determination of circulating immune complexes (CIC) was adapted for flow cytometric analysis. Human granulocytes were used to phagocytose IgG-bearing CIC of serum from systemic lupus erythematosus (SLE) patients. A method for labeling the phagocytosed CIC with FITC-conjugated anti-human IgG was developed where the granulocytes remain in suspension during fixation and labeling. The fluorescence per cell, measured with a flow cytometer, is a measure of the total amount of the phagocytosed IgG. The results indicate that a rapid and quantitative method for the detection and measurement of phagocytosed CIC is possible using the flow cytometer.
Item Type:	Article
Copyright:	© 1985 Wiley
Faculty:	Science and Technology (TNW)
Research Group:	Medical Cell BioPhysics (MCBP)
Link to this item:	http://purl.utwente.nl/publications/60746
Official URL:	http://dx.doi.org/10.1002/cyto.990060407
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