A single platform image cytometer for resource-poor settings to monitor disease progression in HIV infection

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Ymeti, Aurel and Li, Xiao and Lunter, Björn and Breukers, Christian and Tibbe, Arjan G.J. and Terstappen, Leon W.M.M. and Greve, Jan (2007) A single platform image cytometer for resource-poor settings to monitor disease progression in HIV infection. Cytometry Part A, 71A (3). pp. 132-142. ISSN 0196-4763

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Abstract:Background:
For resource-poor countries, affordable methods are required for enumeration of CD4+ T lymphocytes of HIV-positive patients. For infants, additional determination of CD4/CD8 ratio is needed. -
Methods:
We determine the CD4+ and CD8+ T lymphocytes as the CD3+CD4+ and CD3+CD8+ population of blood cells. Target cells are CD3-immunomagnetically separated from the whole blood, and CD4-Phycoerythrin and CD8-PerCP immunofluorescently labeled. A point-of-care single platform image cytometer was developed to enumerate the target CD3+CD4+ and CD3+CD8+ populations. It has light-emitting diodes illumination, is fully computer-controlled, operates from a 12 V battery, and was designed to be cheap and easy-to-handle. Target cells are imaged on a CCD camera and enumerated by an image analysis algorithm. The cytometer outputs the absolute number of CD4+ and CD8+ T lymphocytes/l and CD4/CD8 ratio. -
Results:
The quality of the cell images obtained with the cytometer is sufficient for a reliable enumeration of target cells. The image cytometer achieves an accuracy of better than 10% in the range of 50-1700 cells/l. Analysis of blood samples from HIV patients yields a good agreement with the TruCount method for CD4 and CD8 count and CD4/CD8 ratio. -
Conclusions:
The image cytometer is affordable (component costs $3,000), compact (25 × 25 × 20 cm3), and uses disposable test materials, making it a good candidate to monitor progression of immunodeficiency disease in resource-poor settings. [math mode missing closing $]
Item Type:Article
Copyright:© 2007 Wiley-Liss
Faculty:
Science and Technology (TNW)
Research Group:
Link to this item:http://purl.utwente.nl/publications/60742
Official URL:http://dx.doi.org/10.1002/cyto.a.20375
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