Cluster analysis of flow cytometric list mode data on a personal computer

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Bakker Schut, Tom C. and Grooth de, Bart G. and Greve, Jan (1993) Cluster analysis of flow cytometric list mode data on a personal computer. Cytometry Part A, 14 (6). pp. 649-659. ISSN 0196-4763

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Abstract:A cluster analysis algorithm, dedicated to analysis of flow cytometric data is described. The algorithm is written in Pascal and implemented on an MS-DOS personal computer. It uses k-means, initialized with a large number of seed points, followed by a modified nearest neighbor technique to reduce the large number of subclusters. Thus we combine the advantage of the k-means (speed) with that of the nearest neighbor technique (accuracy). In order to achieve a rapid analysis, no complex data transformations such as principal components analysis were used.
Results of the cluster analysis on both real and artificial flow cytometric data are presented and discussed. The results show that it is possible to get very good cluster analysis partitions, which compare favorably with manually gated analysis in both time and in reliability, using a personal computer.
Item Type:Article
Copyright:© 1993 Wiley-Liss
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Link to this item:http://purl.utwente.nl/publications/60727
Official URL:http://dx.doi.org/10.1002/cyto.990140609
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