Changes in actin organization during the cytotoxic process


Radosevic, Katarina and Leeuwen, Anne Marie T. van and Segers-Nolten, Ine M.J. and Figdor, Carl G. and Grooth, Bart G. de and Greve, Jan (1994) Changes in actin organization during the cytotoxic process. Cytometry Part A, 15 (4). pp. 320-326. ISSN 0196-4763

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Abstract:Changes in organization of F-actin during the cytotoxic process between NK and K562 cells have been observed and studied using confpcal laser scanning microscopy and quantitative fluorescence microscopy. An increase in F-actin content and orientation of F-actin towards the target cell have been observed in, conjugated NK cells. The increase in F-actin content probably reflects activation of the NK cell for the killing process. An increase in F-actin content in the conjugated K562 cell, occurring simultaneously with the appearance of filamentous actin structures that often originated/ended at the contact place with the NK cell, was also observed. These changes were delayed compared to the increase in F-actin content in the NK cell and were accompanied by increasing cytotoxic activity. This indicates that they were results of the interaction of the K562 cell with the activated NK cell. The possible role of target cell microfilaments in the cytotoxic process is addressed.
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Copyright:© 1994 Wiley-Liss
Science and Technology (TNW)
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