Mixed matrix membranes : a new platform for enzymatic reactions


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Sousa André, Joao Miguel de (2009) Mixed matrix membranes : a new platform for enzymatic reactions. thesis.

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Abstract:Glucose oxidase (GOx) was covalently bound onto Eupergit® C particles embedded
in mixed matrix hollow fiber membranes prepared using polyethylene
vinyl alcohol (EVAL) as the polymeric macroporous matrix. The Eupergit
® C particles were chemically modified with ethylenediamine and activated
with glutaraldehyde, ground and fractionated into 20-40 and < 20 μm
size classes. GOx was immobilized in the fibers in dynamic mode. Fibers
containing the smallest particles show the highest immobilization capacity
due to the increased external particle surface area. The activity of immobilized
enzyme was evaluated by dynamic conversion of glucose. The results
proved that the enzyme retains enzymatic activity after immobilization. The
highest glucose conversion yields are obtained with fibers which contain the
bigger particles, which is attributed to a better fiber wall morphology leading
to an improved flow distribution. The results further showed that GOx
covalently immobilized in EVAL/Eupergit® fibers has a lower enzymatic activity
than GOx that is physically immobilized in polyether sulfone (PES)
fibers containing strong cation exchange (SCIEX) resins. The reduced activity
is explained by the differences in the materials used, immobilization
methods, fiber morphologies and applied pH’s in each case.
Item Type:Thesis
Faculty:
Science and Technology (TNW)
Research Group:
Link to this item:http://purl.utwente.nl/publications/60605
Official URL:http://dx.doi.org/10.3990/1.9789036527903
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