Synchronized, Continuous-Flow Zone Electrophoresis

Zalewski, Dawid R. and Kohlheyer, Dietrich and Schlautmann, Stefan and Gardeniers, Han J.G.E. (2008) Synchronized, Continuous-Flow Zone Electrophoresis. Analytical Chemistry, 80 (16). pp. 6228-6234. ISSN 0003-2700

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Abstract:	A new method for performing continuous electrophoretic separation of complex mixtures in microscale devices is proposed. Unlike in free-flow electrophoresis devices, no mechanical pumping is requiredboth fluid transport and separation are driven electrokinetically. This gives the method great potential for on-a-chip integration in multistep analytical systems. The method enables us to collect fractionated sample and tensfold purification is possible. The model of the operation is presented and a detailed description of the optimal conditions for performing purification is given. The chip devices with 10-μm-deep separation chamber of 1.5 mm × 4 mm in size were fabricated in glass. A standard microchip electrophoresis setup was used. Continuous separation of rhodamine B, rhodamine 6G, and fluorescein was accomplished. Purification was demonstrated on a mixture containing rhodamine B and fluorescein, and the recovery of both fractions was achieved. The results show the feasibility of the method.
Item Type:	Article
Copyright:	© 2008 American Chemical Society
Faculty:	Science and Technology (TNW)
Research Group:	Energy, Materials and Systems (EMS) Mesoscale Chemical Systems (MCS)
Link to this item:	http://purl.utwente.nl/publications/60356
Official URL:	http://dx.doi.org/10.1021/ac800567n
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