Sol-gel immobilization of serine proteases for application in organic solvents

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Unen, Dirk-Jan van and Engbersen, Johan F.J. and Reinhoudt, David N. (2001) Sol-gel immobilization of serine proteases for application in organic solvents. Biotechnology and Bioengineering, 75 (2). pp. 154-158. ISSN 0006-3592

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Abstract:The serine proteases α-chymotrypsin, trypsin, and subtilisin Carlsberg were immobilized in a sol-gel matrix and the effects on the enzyme activity in organic media are evaluated. The percentage of immobilized enzyme is 90% in the case of α-chymotrypsin and the resulting specific enzyme activity in the transesterification of N-acetyl-L-phenylalanine ethyl ester with 1-propanol in cyclohexane is 43 times higher than that of a nonimmobilized lyophilized α-chymotrypsin. The activities of trypsin and subtilisin Carlsberg are enhanced with 437 and 31 times, respectively. The effect of immobilization on the enzyme activity is highest in hydrophobic solvents.
Item Type:Article
Copyright:© 2001 Wiley
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Science and Technology (TNW)
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Link to this item:http://purl.utwente.nl/publications/59871
Official URL:http://dx.doi.org/10.1002/bit.1173
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