Confocal restricted-height imaging of suspension cells (CRISC) in a PDMS microdevice during apoptosis

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Munoz-Pinedo, Cristina and Green, Douglas R. and Berg van den, Albert (2005) Confocal restricted-height imaging of suspension cells (CRISC) in a PDMS microdevice during apoptosis. Lab on a Chip, 2005 (5). pp. 628-633. ISSN 1473-0197

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Abstract:We have monitored and imaged cell death induced in human leukemic U937 cells over time using three-color confocal imaging. Three different apoptotic inducers, anti-Fas, TNF- and Etoposide were used. Individual cascaded events such as loss of mitochondrial transmembrane potential, exposure of phosphatidyl-serine, membrane blebbing and permeabilization of the cell membrane have been observed in real time with different individual cells. From the results, an interesting heterogeneicity in the apoptotic phenotype has been observed. The CRISC method is easy to use and provides biologist with a powerful additional tool to study in real-time processes of several hours of duration such as apoptosis. We predict that the period of cell viability obtained after protein coating of the PDMS devices (>80 h) will also allow monitoring of other biological processes of longer duration or long onset time, such as mitosis, phagocytosis and differentiation.
Item Type:Article
Copyright:© The Royal Society of Chemistry 2008
Faculty:
Electrical Engineering, Mathematics and Computer Science (EEMCS)
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Link to this item:http://purl.utwente.nl/publications/59477
Official URL:http://dx.doi.org/10.1039/b503770k
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