Transfer Printing of DNA by “Click” Chemistry


Rozkiewicz, Dorota I. and Gierlich, Johannes and Burley, Glenn A. and Gutschmiedl, Katrin and Carell, Thomas and Ravoo, Bart Jan and Reinhoudt, David N. (2007) Transfer Printing of DNA by “Click” Chemistry. ChemBioChem, 8 (16). pp. 1997-2002. ISSN 1439-4227

[img] PDF
Restricted to UT campus only
: Request a copy
Abstract:This paper describes a straightforward procedure to immobilize oligonucleotides on glass substrates in well-defined micropatterns by microcontact printing with a dendrimer-modified stamp. The oligonucleotides are efficiently immobilized by click chemistry induced by microcontact printing. Acetylene-modified oligonucleotides were treated with an azide-terminated glass slide under the confinement of the dendrimer-modified stamp, without the use of a CuI catalyst. The immobilization is an irreversible, covalent, and one-step reaction that results in stable attachment of the oligonucleotides. Oligonucleotides with the acetylene-modification at the 5 terminus hybridize selectively with full-length, complementary targets. Strands with more than one acetylene linker do not hybridize with complementary strands.
Item Type:Article
Copyright:© 2007 WILEY-VCH Verlag
Research Group:
Link to this item:
Official URL:
Export this item as:BibTeX
HTML Citation
Reference Manager


Repository Staff Only: item control page

Metis ID: 243315