Methods and instrumentation for quantitative microchip capillary electrophoresis


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Revermann, Tobias (2007) Methods and instrumentation for quantitative microchip capillary electrophoresis. thesis.

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Abstract:The development of novel instrumentation and analytical methodology for
quantitative microchip capillary electrophoresis (MCE) is described in this
thesis. Demanding only small quantities of reagents and samples,
microfluidic instrumentation is highly advantageous. Fast separations at high
voltages have been performed, which require the use of flexible high-voltage
power supplies that have been developed in this work.
An introductory overview of the general requirements of MCE is provided.
Differences of chip-based CE and conventional CE are addressed, and the
effects of buffer decomposition, reactions at electrodes, clogging of
microchips, surface interactions, injection and power supply as well as
detector-related problems on measurements are discussed. Additionally also
strategies for proper analysis and technical solutions are identified.
Item Type:Thesis
Research Group:
Link to this item:http://purl.utwente.nl/publications/58983
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