Ultra-high-speed imaging of bubbles interacting with cells and tissue

Share/Save/Bookmark

Versluis, Michel and Marmottant, Philippe and Hilgenfeldt, Sascha and Ohl, Claus-Dieter and Chin, Chien T. and Wamel van, Annemieke and Jong de, Nico and Lohse, Detlef (2006) Ultra-high-speed imaging of bubbles interacting with cells and tissue. Journal of the Acoustical Society of America, 120 . p. 3229. ISSN 00012097

[img]
Preview
PDF
558Kb
Abstract:Ultrasound contrast microbubbles are exploited in molecular imaging, where bubbles are directed to target cells and where their high-scattering cross section to ultrasound allows for the detection of pathologies at a molecular level. In therapeutic applications vibrating bubbles close to cells may alter the permeability of cell membranes, and these systems are therefore highly interesting for drug and gene delivery applications using ultrasound. In a more extreme regime bubbles are driven through shock waves to sonoporate or kill cells through intense stresses or jets following inertial bubble collapse. Here, we elucidate some of the underlying mechanisms using the 25-Mfps camera Brandaris128, resolving the bubble dynamics and its interactions with cells. We quantify acoustic microstreaming around oscillating bubbles close to rigid walls and evaluate the shear stresses on nonadherent cells. In a study on the fluid dynamical interaction of cavitation bubbles with adherent cells, we find that the nonspherical collapse of bubbles is responsible for cell detachment. We also visualized the dynamics of vibrating microbubbles in contact with endothelial cells followed by fluorescent imaging of the transport of propidium iodide, used as a membrane integrity probe, into these cells showing a direct correlation between cell deformation and cell membrane permeability.
Item Type:Article
Copyright:© 2006 Acoustical Society of America
Faculty:
Science and Technology (TNW)
Research Group:
Link to this item:http://purl.utwente.nl/publications/58162
Export this item as:BibTeX
EndNote
HTML Citation
Reference Manager

 

Repository Staff Only: item control page