Heparinized crosslinked collagen structures for the expansion and differentiation of hematopoietic stem cells


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Siebum, Bastiaan (2007) Heparinized crosslinked collagen structures for the expansion and differentiation of hematopoietic stem cells. thesis.

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Abstract:It is generally accepted that bone marrow contains niches that regulate the activity of hematopoietic stem cells (HSCs). These cells are the source of all different blood
cells. In their niche the HSCs are localized in a specific microenvironment, where they interact with stromal cells, extracellular matrix (ECM) components and molecules like cytokines, chemokines and growth factors.
The objective of the studies presented in this thesis was to develop a bioreactor system, in which the bone marrow is mimicked, for the controlled expansion and
differentiation of HSCs and hematopoietic progenitor cells (HPCs). Such a system is expected to facilitate an effective expansion of HSCs and to provide a model for
investigating the mechanisms of hematopoiesis and processes such as stem cell mobilization and homing. The in vitro expansion and controlled differentiation of
HSCs and HPCs is of great interest for application in bone marrow transplantation.
Additionally, controlled differentiation of these cells can be used to produce blood cells for transfusion. The focus will be on the expansion of HPCs and their
differentiation into erythroid cells.
Item Type:Thesis
Link to this item:http://purl.utwente.nl/publications/58040
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