Colloidal carbon particles as a new label for rapid immunochemical test methods: quantitative computer image analysis of results

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Amerongen, A. van and Wichers, J.H. and Berendsen, L.B.J.M. and Timmermans, A.J.M. and Keizer, G.D. and Doorn, A.W.J. van and Bantjes, A. and Gelder, W.M.J. van (1993) Colloidal carbon particles as a new label for rapid immunochemical test methods: quantitative computer image analysis of results. Journal of Biotechnology, 30 (2). pp. 185-195. ISSN 0168-1656

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Abstract:Colloidal carbon particles can serve as label in sol particle immunoassays. The universal applicability of these particles in qualitative and (semi)quantitative immunoassays has been demonstrated. Sol particle and/or dipstick immunoassays, not yet optimized in terms of sensitivity, are discussed.

The colloidal label has been used successfully in a mouse immunoglobulin isotyping kit. Human serum albumin spotted onto nitrocellulose in a concentration range of 7.8 to 1000 ng could be detected using anti-albumin antibody adsorbed onto colloidal carbon particles. It was also possible to perform a competitive assay with this conjugate for a concentration range of free human serum albumin varying from 0.25 to 6.75 ¿g. The Kunitz-type trypsin inhibitor from soybean was determined by a colloidal carbon based immunoassay in a range of 2.5 to 160 ng. In this assay, free and colloidal carbon-bound inhibitor competed for binding specific antibodies spotted onto a nitrocellulose membrane.

An image- and data-processing procedure has been developed that enables a rapid and simple quantification of colloidal carbon sol particle immunoassays. The average grey level of a spot is taken as a measure for quantitative purposes. This so-called Sol-particle Image Processed ImmunoAssay (SIPIA) procedure is equally well applicable to assays using other colloidal particles.
Item Type:Article
Copyright:© 1993 Elsevier Science
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Link to this item:http://purl.utwente.nl/publications/57415
Official URL:http://dx.doi.org/10.1016/0168-1656(93)90112-Z
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