Affinity membranes for hormone removal from aqueous solutions


Urmenyi, A.M. and Poot, A.A. and Wessling, M. and Mulder, M.H.V. (2005) Affinity membranes for hormone removal from aqueous solutions. Journal of Membrane Science, 259 (1-2). pp. 91-102. ISSN 0376-7388

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Abstract:A novel affinity membrane was prepared by covalent binding of antibodies (against 17--estradiol) to a micro-porous poly(ethylene vinyl
alcohol) (EVAL) membrane, taking benefit from the high surface area of EVAL membranes and the large number of reactive groups available
for further surface modification. The covalent coupling of the antibody occurred via its non-specific chain to maximize the number of
available binding sites for hormones. To achieve this site-oriented coupling first poly(ethylene glycol) bis-hydrazide (PEG-Hz) was reacted
with glutaraldehyde-modified EVAL membrane, followed by coupling of oxidized antibody to the PEG-Hz spacer arm. Confocal microscopy
demonstrated that most of the antibodies were grafted on the outer surface, rather than inside the membrane. In dynamic filtration, 17--
estradiol was retained for 99%. The specificity of the membrane was demonstrated by its capability to distinguish 17--estradiol from the
nearly identical compound estrone.
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Copyright:© 2005 Elsevier
Science and Technology (TNW)
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