Monitoring of yeast cell concentration using a micromachined impedance sensor


Krommenhoek, E.E. and Gardeniers, J.G.E. and Bomer, J.G. and Berg, A. van den and Li, X. and Ottens, M. and Wielen, L.A.M. van der and Dedem, G.W.K. van and Leeuwen, M. and Gulik, W.M. van and Heijnen, J.J. (2005) Monitoring of yeast cell concentration using a micromachined impedance sensor. In: 13th International Conference on Solid-StateSensors, Actuators and Microsystems, Transducers 2005, June 5-9, 2005, Seoul, Korea (pp. pp. 283-286).

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Abstract:The paper describes the design, modelling and experimental characterization of a micromachined impedance sensor for on-line monitoring of the viable yeast cell concentration (biomass) in a miniaturized cell assay. Measurements in a Saccharomyces cerevisiae cell culture show that the permittivity of the cell suspension depends linearly on the biomass concentration within the range of 0 to 9 g/l. In order to compensate the measurements for changes in the dielectric properties of the background electrolyte, the use of a three-electrode configuration in combination with a semi-permeable pHEMA membrane was explored. Measurements showed that the impedance of the hydrated pHEMA varies with only the background electrolyte conductivity, and not with the concentration of cells, indicating that pHEMA is suitable for this purpose. The optimal pHEMA membrane thickness was determined using finite element modelling.
Item Type:Conference or Workshop Item
Copyright:© 2005 IEEE
Science and Technology (TNW)
Electrical Engineering, Mathematics and Computer Science (EEMCS)
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