The potential of autofluorescence for the detection of single living cells for label-free cell sorting in microfluidic systems

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Emmelkamp, Jurjen and Wolbers, Floor and Andersson, Helene and DaCosta, Ralph S. and Wilson, Brian C. and Vermes, István and Berg van den, Albert (2004) The potential of autofluorescence for the detection of single living cells for label-free cell sorting in microfluidic systems. Electrophoresis, 25 (21-22). pp. 3740-3745. ISSN 0173-0835

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Abstract:A novel method for studying unlabeled living mammalian cells based on their autofluorescence (AF) signal in a prototype microfluidic device is presented. When combined, cellular AF detection and microfluidic devices have the potential to facilitate high-throughput analysis of different cell populations. To demonstrate this, unlabeled cultured cells in microfluidic devices were excited with a 488 nm excitation light and the AF emission (> 505 nm) was detected using a confocal fluorescence microscope (CFM). For example, a simple microfluidic three-port glass microstructure was used together with conventional electroosmotic flow (EOF) to switch the direction of the fluid flow. As a means to test the potential of AF-based cell sorting in this microfluidic device, granulocytes were successfully differentiated from human red blood cells (RBCs) based on differences in AF. This study demonstrated the use of a simple microfabricated device to perform high-throughput live cell detection and differentiation without the need for cell-specific fluorescent labeling dyes and thereby reducing the sample preparation time. Hence, the combined use of microfluidic devices and cell AF may have many applications in single-cell analysis.
Item Type:Article
Copyright:© 2004 WILEY-VCH Verlag
Faculty:
Electrical Engineering, Mathematics and Computer Science (EEMCS)
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Link to this item:http://purl.utwente.nl/publications/48307
Official URL:http://dx.doi.org/10.1002/elps.200406070
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