Multistep Energy Transfer in Single Molecular Photonic Wires


Heilemann, Mike and Tinnefeld, Philip and Sanchez Mosteiro, Gabriel and Garcia-Parajo, Maria F. and Hulst, Niek F. van and Sauer, Markus (2004) Multistep Energy Transfer in Single Molecular Photonic Wires. Journal of the American Chemical Society, 126 (21). pp. 6514-6515. ISSN 0002-7863

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Abstract:We demonstrate the synthesis and spectroscopic characterization of an unidirectional photonic wire based on four highly efficient fluorescence energy-transfer steps (FRET) between five spectrally different chromophores covalently attached to double-stranded DNA. The DNA-based modular conception enables the introduction of various chromophores at well-defined positions and arbitrary interchromophore distances. While ensemble fluorescence measurements show overall FRET efficiencies between 15 and 30%, single-molecule spectroscopy performed on four spectrally separated detectors easily uncovers subpopulations that exhibit overall FRET efficiencies of up to ~90% across a distance of 13.6 nm and a spectral range of ~200 nm. Fluorescence trajectories of individual photonic wires show five different fluorescence intensity patterns which can be ascribed to successive photobleaching events.
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Copyright:© 2004 American Chemical Society
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