Multistep Energy Transfer in Single Molecular Photonic Wires

Heilemann, Mike and Tinnefeld, Philip and Sanchez Mosteiro, Gabriel and Garcia-Parajo, Maria F. and Hulst van, Niek F. and Sauer, Markus (2004) Multistep Energy Transfer in Single Molecular Photonic Wires. Journal of the American Chemical Society, 126 (21). pp. 6514-6515. ISSN 0002-7863

PDF
Restricted to UT campus only: Request a copy
88Kb

Abstract:	We demonstrate the synthesis and spectroscopic characterization of an unidirectional photonic wire based on four highly efficient fluorescence energy-transfer steps (FRET) between five spectrally different chromophores covalently attached to double-stranded DNA. The DNA-based modular conception enables the introduction of various chromophores at well-defined positions and arbitrary interchromophore distances. While ensemble fluorescence measurements show overall FRET efficiencies between 15 and 30%, single-molecule spectroscopy performed on four spectrally separated detectors easily uncovers subpopulations that exhibit overall FRET efficiencies of up to ~90% across a distance of 13.6 nm and a spectral range of ~200 nm. Fluorescence trajectories of individual photonic wires show five different fluorescence intensity patterns which can be ascribed to successive photobleaching events.
Item Type:	Article
Copyright:	© 2004 American Chemical Society
Research Group:	Optical Sciences (OS)
Link to this item:	http://purl.utwente.nl/publications/47574
Official URL:	http://dx.doi.org/10.1021/ja049351u
Export this item as:	BibTeX EndNote HTML Citation Reference Manager

Show download statistics for this publication

Repository Staff Only: item control page

Metis ID: 218155