On-Chip Hydrodynamic Chromatography Separation and Detection of Nanoparticles and Biomolecules

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Blom, Marko T. and Chmela, Emil and Oosterbroek, R. Edwin and Tijssen, Robert and Berg, Albert van den (2003) On-Chip Hydrodynamic Chromatography Separation and Detection of Nanoparticles and Biomolecules. Analytical Chemistry, 75 (24). pp. 6761-6768. ISSN 0003-2700

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Abstract:For the first time, on-chip planar hydrodynamic chromatography is combined with UV absorption detection. This technique is suitable for size characterization of synthetic polymers, biopolymers, and particles. Possible advantages of an on-chip hydrodynamic chromatography system over conventional techniques, such as size exclusion chromatography, and field-flow fractionation are fast analysis, high efficiency, reduced solvent consumption, and easy temperature control. The hydrodynamic separations are performed in a planar configuration realized in fused silica using a mixture of fluorescent and nonfluorescent polystyrene particles with sizes ranging from 26 to 155 nm. The planar chip configuration consists of a 1-μm-high, 0.5-mm-wide, and 69-mm-long channel, an integrated 150-pL injection structure, and a 30-μm-deep and 30-μm-wide detection cell, suitable for UV absorption detection. By combination of the separation data obtained in the new fused-silica chip with those obtained using a previously presented planar hydrodynamic chromatography chip, which was realized using silicon and glass microtechnology, a description of the retention and dispersion behavior of planar hydrodynamic chromatography is obtained. Especially the influence of the sidewalls on the dispersion is investigated. Furthermore a hydrodynamic separation within 70 s of several biopolymers is shown in the glass−silicon chip.
Item Type:Article
Copyright:© 2003 American Chemical Society
Faculty:
Electrical Engineering, Mathematics and Computer Science (EEMCS)
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Link to this item:http://purl.utwente.nl/publications/46138
Official URL:http://dx.doi.org/10.1021/ac034663l
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