Adhesion and growth of electrically-active cortical neurons on polyethyleimine patterns microprinted on PEO-PPO-PEO triblockcopolymer-coated hydrophobic surfaces

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Ruardij, T.G. and Boogaart van den, M.A.F. and Rutten, W.L.C. (2002) Adhesion and growth of electrically-active cortical neurons on polyethyleimine patterns microprinted on PEO-PPO-PEO triblockcopolymer-coated hydrophobic surfaces. IEEE transactions on nanobioscience, 1 (1). pp. 1-8. ISSN 15361241

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Abstract:This paper describes the adhesion and growth of dissociated cortical neurons on chemically patterned surfaces over a time period of 30 days. The presence of neurons was demonstrated by measurement of spontaneous bioelectrical activity on a micropatterned multielectrode array. Chemical patterns were prepared with a combination of neurophobic layers of polyethylenoxide-polypropylenoxide-polyethylenoxide (PEO-PPO-PEO) triblockcopolymers adsorbed onto hydrophobic surfaces and neurophilic microprinted tracks of polyethylenimine (PEI). Results showed that commercially available PEO-PPO-PEO triblockcopolymers F108 and F127 (Synperonics, ICI) significantly reduced the adhesion of neuronal tissue when adsorbed on hydrophobic Polyimide (PI) and Fluorocarbon (FC) surfaces over a time period of eight days. In general, both F108- and F127-coated PI displayed equal or better neurophobic background properties after 30 days. Viability of neuronal tissue after 30 days on PEI microprinted F108- and F127-coated PI was comparable with relatively high viability factors between 0.9 and 1 (scale from 0 to 1). Summarizing, the strategy to combine the neurophobic adsorbed triblock-copolymers F108 and F127 onto hydrophobic surfaces with neurophilic microprinted PEI resulted in relatively long-term neuronal pattern preservation with high numbers of viable neurons present after 30 days.
Item Type:Article
Copyright:©2002 IEEE
Faculty:
Electrical Engineering, Mathematics and Computer Science (EEMCS)
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Link to this item:http://purl.utwente.nl/publications/43920
Official URL:http://dx.doi.org/10.1109/TNB.2002.806921
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