Modeled channel distributions explain extracellular recordings from cultured neurons sealed to microelectrodes

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Buitenweg, Jan Reinoud and Rutten, Wim L.C. and Marani, Enrico (2002) Modeled channel distributions explain extracellular recordings from cultured neurons sealed to microelectrodes. IEEE Transactions on Biomedical Engineering, 49 (11). pp. 1580-1590. ISSN 0018-9294

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Abstract:Amplitudes and shapes of extracellular recordings from single neurons cultured on a substrate embedded microelectrode depend not only on the volume conducting properties of the neuron-electrode interface, but might also depend on the distribution of voltage-sensitive channels over the neuronal membrane. In this paper, finite-element modeling is used to quantify the effect of these channel distributions on the neuron-electrode contact. Slight accumulation or depletion of voltage-sensitive channels in the sealing membrane of the neuron results in various shapes and amplitudes of simulated extracellular recordings. However, estimation of channel-specific accumulation factors from extracellular recordings can be obstructed by co-occuring ion currents and defect sealing. Experimental data from cultured neuron-electrode interfaces suggest depletion of sodium channels and accumulation of potassium channels.
Item Type:Article
Copyright:©2002 IEEE
Faculty:
Electrical Engineering, Mathematics and Computer Science (EEMCS)
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Link to this item:http://purl.utwente.nl/publications/43903
Official URL:http://dx.doi.org/10.1109/TBME.2002.805555
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