Dielectrophoretic trapping of dissociated fetal cortical rat neurons

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Heida, Tjitske and Rutten, Wim L.C. and Marani, Enrico (2001) Dielectrophoretic trapping of dissociated fetal cortical rat neurons. IEEE Transactions on Biomedical Engineering, 48 (8). pp. 921-930. ISSN 0018-9294

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Abstract:Recording and stimulating neuronal activity at multiple sites can be realized with planar microelectrode arrays. Efficient use of such arrays requires each site to be covered by at least one neuron. By application of dielectrophoresis (DEP), neurons can be trapped onto these sites. This study investigates negative dielectrophoretic trapping of fetal cortical rat neurons. A planar quadrupole microelectrode structure was used for the creation of a nonuniform electric field. The field was varied in amplitude (1, 3, and 5 V) and frequency (10 kHz-50 MHz). Experimental results were compared with a theoretical model to investigate the yield (the number of neurons trapped in the center of the electrode structure) with respect to time, amplitude and frequency of the field. The yield was a function of time1/3 according to theory. However, unlike the model predicted, an amplitude-dependent frequency behavior was present and unexpected peaks occurred in the DEP-spectra above 1 MHz. Gain/phase measurements showed a rather unpredictable behavior of the electrode plate above 1 MHz, and temperature measurement showed that heating of the medium influenced the trapping effect, especially for larger amplitudes and higher frequencies
Item Type:Article
Copyright:©2001 IEEE
Faculty:
Electrical Engineering, Mathematics and Computer Science (EEMCS)
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Link to this item:http://purl.utwente.nl/publications/42126
Official URL:http://dx.doi.org/10.1109/10.936368
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Metis ID: 200459