The effects of crown ethers on the activity of enzymes in organic solvents


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Unen van, Dirk-Jan and Engbersen, Johan F.J. and Reinhoudt, David N. (2001) The effects of crown ethers on the activity of enzymes in organic solvents. In: Enzymes in Nonaqueous Solvents: Methods and Protocols. Humana Press, Totowa, New Jersey, pp. 213-225. ISBN 9780896039292

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Abstract:Currently, the applicability of enzymes in synthetic organic chemistry is well recognized. The field of enzyme-catalyzed organic synthesis has been further boosted by the recognition that enzymes can operate in organic solvents. The use of nonaqueous media for enzymatic conversions offers a number of advantages, like enhanced thermal stability of the enzyme, increased substrate solubility, a shift of the equilibrium in favor of synthesis over hydrolysis, and altered selectivity properties of the enzyme (1). However, the most important drawback of the use of enzymes in organic media is the reduced catalytic activity compared to aqueous conditions. Typically, this reduction in activity is two to six orders of magnitude (2). However, studies in our laboratory have revealed that (pre)treatment of the enzymes with crown ethers can enhance enzyme activities in nonaqueous organic solvents up to a level approaching the activity under aqueous conditions.
Item Type:Book Section
Copyright:© 2001 Springer
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Science and Technology (TNW)
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Link to this item:http://purl.utwente.nl/publications/36699
Official URL:http://dx.doi.org/10.1385/1-59259-112-4:213
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