Cell biology beyond the diffraction limit: near-field scanning optical microscopy


Lange, Frank de and Cambi, Alessandra and Huijbens, Richard and Bakker, Bärbel I. de and Rensen, Wouter and Garcia-Parajo, Maria and Hulst, Niek van and Figdor, Carl G. (2001) Cell biology beyond the diffraction limit: near-field scanning optical microscopy. Journal of Cell Science, 114 . pp. 4153-4160. ISSN 0021-9533

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Abstract:Throughout the years, fluorescence microscopy has proven to be an extremely versatile tool for cell biologists to study live cells. Its high sensitivity and non-invasiveness, together with the ever-growing spectrum of sophisticated fluorescent indicators, ensure that it will continue to have a prominent role in the future. A drawback of light microscopy is the fundamental limit of the attainable spatial resolution - similar to 250 urn - dictated by the laws of diffraction. The challenge to break this diffraction limit has led to the development of several novel imaging techniques. o­ne of them, near-field scanning optical microscopy (NSOM), allows fluorescence imaging at a resolution of o­nly a few tens of nanometers and, because of the extremely small near-field excitation volume, reduces background fluorescence from the cytoplasm to the extent that single-molecule detection sensitivity becomes within reach. NSOM allows detection of individual fluorescent proteins as part of multimolecular complexes o­n the surface of fixed cells, and similar results should be achievable under physiological conditions in the near future
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Link to this item:http://purl.utwente.nl/publications/36643
Official URL:http://jcs.biologists.org/cgi/content/full/114/23/4153
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