A flow cytometric study of the membrane potential of natural killer and k562 cells during the cytotoxic process

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Radosevic, Katarina and Bakker Schut, Tom C. and Graft van, Marja and Grooth de, Bart G. and Greve, Jan (1993) A flow cytometric study of the membrane potential of natural killer and k562 cells during the cytotoxic process. Journal of Immunological Methods, 161 (1). pp. 119-128. ISSN 0022-1759

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Abstract:This study demonstrates that it is possible to investigate the membrane potential of interacting cells during the cytotoxic process using flow cytometry. Changes in the membrane potential of NK and K562 cells, involved in a cell-mediated cytotoxic process, were studied by standard and slit-scan flow cytometry, using the membrane potential sensitive fluorescent probe DiBAC4(3). The NK cells were labeled with a membrane marker (TR-18 or DiI) prior to incubation with K562 cells and the conjugates that were formed could be identified on the basis of the membrane marker fluorescence and light scattering signals. With a slit-scan technique we measured the membrane potential of each cell in a conjugate separately. The results show that depolarization of the K562 cell occurs as a consequence of the cytotoxic activity of the NK cell. This depolarization appears to be an early sign of cell damage because the cell membrane still remains impermeable to propidium iodide. Our data also indicate that depolarization of the NK cell occurs as a result of its cytotoxic activity.
Item Type:Article
Copyright:© 1993 Elsevier Science
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Link to this item:http://purl.utwente.nl/publications/24587
Official URL:http://dx.doi.org/10.1016/0022-1759(93)90203-J
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Metis ID: 129388